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GFAP Protein (AA 1-430) (His-IF2DI Tag)

This Recombinant GFAP protein is expressed in Escherichia coli (E. coli).
Catalog No. ABIN7123095

Quick Overview for GFAP Protein (AA 1-430) (His-IF2DI Tag) (ABIN7123095)

Target

See all GFAP Proteins
GFAP (Glial Fibrillary Acidic Protein (GFAP))

Protein Type

Recombinant

Origin

  • 12
  • 9
  • 5
  • 4
  • 2
  • 1
  • 1
  • 1
Mouse

Source

  • 17
  • 5
  • 5
  • 2
  • 2
  • 1
Escherichia coli (E. coli)

Application

Western Blotting (WB), ELISA

Purity

Greater than 90 % as determined by SDS-PAGE.
  • Protein Characteristics

    AA 1-430

    Purification tag / Conjugate

    This GFAP protein is labelled with His-IF2DI Tag.

    Purpose

    Recombinant Mouse GFAP

    Sterility

    0.2 μm filtered
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  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Comment

    IF2DI tag is a small molecule soluble label from escherichia coli domain I of translation initiation factor IF2 which molecular weight is about 18kd.IFD fusion proteins don't compromise their bioactivity that makes it a good substitution of MBP or GST tags.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

    Buffer

    Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

    Storage

    -20 °C,-80 °C

    Storage Comment

    The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

    Expiry Date

    12 months
  • Target

    GFAP (Glial Fibrillary Acidic Protein (GFAP))

    Alternative Name

    GFAP

    Background

    Wu:fb34h11, ALXDRD, cb345, etID36982.3, FLJ42474, FLJ45472, GFAP, GFAP_HUMAN, gfapl, Glial fibrillary acidic protein, Intermediate filament protein, wu:fk42c12, xx:af506734, zgc:110485

    Molecular Weight

    70.5 kDa

    UniProt

    P03995
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