Dihydrofolate Reductase Protein (DHFR) (AA 1-187) (His tag)
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- Target See all Dihydrofolate Reductase (DHFR) Proteins
- Dihydrofolate Reductase (DHFR)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-187
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Dihydrofolate Reductase protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB)
- Purity
- Greater than 95 % as determined by SDS-PAGE
- Sterility
- 0.2 μm filtered
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Dihydrofolate Reductase (DHFR) (AA 1-187) protein (His tag)
DHFR Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Dihydrofolate Reductase (DHFR) (AA 88-187) protein (GST tag)DHFR Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Dihydrofolate Reductase (DHFR) (AA 1-187) protein (GST tag)DHFR Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Dihydrofolate Reductase (DHFR) (AA 2-187) protein (His-SUMO Tag)DHFR Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Dihydrofolate Reductase (DHFR) protein (Myc-DYKDDDDK Tag)DHFR Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial at 10,000 rpm for 1 minute, reconstitute at 200 μg/mL in sterile distilled water by gentle pipetting 2-3 times, don't vortex
- Buffer
- Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C for 12 months as lyophilized, 2-8°C for 1 month under sterile conditions after reconstitution
- Expiry Date
- 12 months
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- Target
- Dihydrofolate Reductase (DHFR)
- Alternative Name
- Dihydrofolate reductase DHFR (DHFR Products)
- Synonyms
- DHFR Protein, F10_orf160 Protein, ECK0049 Protein, JW0047 Protein, tmrA Protein, cb595 Protein, zgc:86637 Protein, DDBDRAFT_0187132 Protein, DDBDRAFT_0230113 Protein, DDB_0187132 Protein, DDB_0230113 Protein, CG14887 Protein, Dmel\\CG14887 Protein, dhfr Protein, DHFRP1 Protein, DYR Protein, 8430436I03Rik Protein, AA607882 Protein, AI662710 Protein, AW555094 Protein, Dhfr1 Protein, confers resistance to sulfonamides Protein, dihydrofolate reductase Protein, dihydrofolate reductase DhfR Protein, Dihydrofolate reductase Protein, dhfr Protein, DHFR Protein, dhfR Protein, folA Protein, dfrA Protein, LOC707382 Protein, CNJ01940 Protein, DDB_G0286755 Protein, Dhfr Protein, Mesil_0096 Protein, Arch_0408 Protein, Fbal_3367 Protein, Ilyop_0482 Protein, Lbys_2007 Protein, Riean_0733 Protein, Mrub_1855 Protein, Poras_0766 Protein, Halhy_3552 Protein, Mesop_3614 Protein, LOC609048 Protein
- Background
- DHFR, DHFRP1, Dihydrofolate reductase, DYR, DYR_HUMAN, EC 1.5.1.3
- Molecular Weight
- 20.5 kDa
- UniProt
- P00375
- Pathways
- Mitotic G1-G1/S Phases
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