ITGAV/ITGB8 Protein (His tag)

Catalog No. ABIN7121506

Product Details

Target: ITGAV/ITGB8 (Integrin alpha V beta 8 (ITGAV/ITGB8))

Protein Type: Recombinant

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This ITGAV/ITGB8 protein is labelled with His tag.

Purpose: Cynomolgus Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein, His Tag&Tag Free

Sequence: Phe 31 - Pro 993 (ITGAV) & Glu 43 - Ser 681 (ITGB8)

Characteristics: Cynomolgus ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free (IT8-C52W9) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Pro 993 (ITGAV) & Glu 43 - Ser 681 (ITGB8) (Accession # A0A2K5WCD3-1 (ITGAV) & G7P0S0-1 (ITGB8).

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: Cynomolgus Integrin alpha V beta 8 (ITGAV&ITGB6) Heterodimer Protein, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 113.1 kDa (ITGAV) and 76.0 kDa (ITGB8). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Glu 43 (ITGB8). The non-reducing (NR) protein migrates as kDa (ITGAV) & 75-85 kDa (ITGB8) respectively due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 50 mM Tris, 150 mM NaCl, pH 7.5

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: ITGAV/ITGB8 (Integrin alpha V beta 8 (ITGAV/ITGB8))

Alternative Name: Integrin alpha V beta 8 (ITGAV&ITGB8) (ITGAV/ITGB8 Products)

Background: Synonyms: Integrin alpha V beta 8,ITGAV&ITGB8,
Description: Integrin alpha V beta 8 (ITGAV & ITGB8 or ITGAVB8) is expressed in yolk sac, placenta, brain perivascular astrocytes, Schwann cells, renal glomerular mesangial cells and pulmonary epithelial cells. Unlike other alpha V integrins, ITGAVB8 does not appear to assume different activation states, and the cytoplasmic tail does not connect to the cytoskeleton. It binds ligands containing an RGD motif, including vitronectin, fibrin and the latency associated peptide (LAP) of the latent TGF-beta complex. High affinity binding of alpha V beta 8 to LAP allows proteolytic cleavage by MT1-MMP, which releases active TGF-beta. This mechanism differs from that of alpha V beta 6, the other alpha V integrin which can activate TGF-beta from latency through non-proteolytic mechanisms. Downstream effects of TGF-beta activation include control of cell growth and associated vascularization.

Molecular Weight: 113.1 kDa (ITGAV) & 76.0 kDa (ITGB8)