Claudin 18.2 protein-VLP (GFP tag)

Catalog No. ABIN7199115

Product Details

Target: Claudin 18.2

Protein Type: VLP

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: GFP tag

Purpose: Fluorescent Human Claudin-18.2 Full Length Protein-VLP (HEK293)

Sequence: Met 1 - Val 261

Characteristics: Fluorescent Human Claudin-18.2 Full Length Protein-VLP (CL2-HF218) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Val 261 (Accession # P56856-2).

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Application Notes: This protein carries a GFP tag at the C-terminus. The protein has a calculated MW of 27.7 kDa.

Comment:

Virus-like particles (VLPs) are formed by self-assembly of envelop/capsid proteins from viruses. Membrance Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.
The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4

Storage: -80 °C

Storage Comment: -70°C

Target Details

Target: Claudin 18.2

Alternative Name: Claudin-18.2

Background: Synonyms: Claudin-18.2,CLDN18,Claudin-18,
Description: Claudins (CLDNs) are a family of proteins that form tight junctions and maintain the polarity of epithelial and endothelial cells. CLDN18 is specifically expressed in the stomach and lung. Of the two CLDN18 isoform transcripts produced by alternative splicing, CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific. CLDN18.2 is a highly selective gastric lineage antigen expressed exclusively on short-lived differentiated gastric epithelial cells where it has only limited accessibility to antibody drugs.14,15 CLDN18.2 is maintained during the course of malignant transformation and thus frequently displayed on the surface of human gastric cancer cells.

Molecular Weight: 27.7 kDa

NCBI Accession: NP_001002026