SARS-CoV-2 Spike Protein (B.1.1.529 - Omicron, Trimer) (His tag)
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- Target See all SARS-CoV-2 Spike Proteins
- SARS-CoV-2 Spike
- Protein Type
- Recombinant
- Protein Characteristics
- B.1.1.529 - Omicron, Trimer
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Origin
- SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Omicron
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SARS-CoV-2 Spike protein is labelled with His tag.
- Application
- ELISA, SDS-PAGE (SDS)
- Purpose
- SARS-CoV-2 Spike Trimer, His Tag (B.1.1.529/Omicron) (MALS verified)
- Characteristics
- SARS-CoV-2 Spike Trimer, His Tag (B.1.1.529/Omicron) is the ectodomain of SARS-CoV-2 spike protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F). The 35 spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: B.1.1.529; GISAID clade: GR/484A; Nextstrain clade: 21K). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. Predicted N-terminus: Val 16
- Purity
- > 95% as determined by SDS-PAGE. > 95% as determined by SEC-MALS.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product SARS-CoV-2 Spike Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
- Handling Advice
- Please avoid repeated freeze-thaw cycles.
- Storage
- -20 °C/-80 °C
- Storage Comment
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For long term storage, the product should be stored at lyophilized state at -20°C or lower.This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 months under sterile conditions after reconstitution. - Expiry Date
- 12 monts
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- Target
- SARS-CoV-2 Spike
- Abstract
- SARS-CoV-2 Spike Products
- Synonyms
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- Target Type
- Viral Protein
- Background
- It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- Molecular Weight
- 138.2 kDa
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