PTK7 Protein (AA 31-704) (His tag,AVI tag,Biotin)

Catalog No. ABIN7013690

Product Details

Target: PTK7 (PTK7 Protein tyrosine Kinase 7 (PTK7))

Protein Type: Recombinant

Protein Characteristics: AA 31-704

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This PTK7 protein is labelled with His tag,AVI tag,Biotin.

Purpose: Biotinylated Human PTK7 / CCK4 protein, His,Avitag™ (MALS verified)

Specificity: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Purity: >95 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Comment:

Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4

Storage: -80 °C

Target Details

Target: PTK7 (PTK7 Protein tyrosine Kinase 7 (PTK7))

Alternative Name: PTK7 (PTK7 Products)

Synonyms: PTK7 Protein, CCK-4 Protein, CCK4 Protein, Srf Protein, 8430404F20Rik Protein, chz Protein, mPTK7/CCK4 Protein, KLG Protein, protein tyrosine kinase 7 (inactive) Protein, protein tyrosine kinase 7 Protein, PTK7 protein tyrosine kinase 7 Protein, PTK7 Protein, Ptk7 Protein

Background: PTK7, highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.

Molecular Weight: 78.2 kDa

NCBI Accession: NP_002812

Pathways: RTK Signaling, Tube Formation