SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (G204R, R203K) (His tag)
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- Target See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) Proteins
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- G204R, R203K
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Origin
- SARS Coronavirus-2 (SARS-CoV-2)
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SARS-CoV-2 Nucleocapsid protein is labelled with His tag.
- Purpose
- SARS-CoV-2 Nucleocapsid protein (R203K, G204R), His Tag
- Sequence
- AA 1-419
- Characteristics
- SARS-CoV-2 Nucleocapsid protein (R203K, G204R), His Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (R203K, G204R). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR)-linker responsible for phosphorylation. The mutations (R203K, G204R) were identified on the SR-linker on the nucleocapsid protein of SARS-CoV-2 variants. Phosphorylation of this SR-link motif in SARS-CoV modulates nucleocapsid multimerization, translational inhibitory activity and cellular localization. The co-occuring mutations R203K and G204R may decrease the overall structural flexibility of SARS-COV-2 N protein.
- Purity
- >90 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product SARS-CoV-2 N Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS, pH 7.4
- Storage
- -20 °C
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- Target
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- Alternative Name
- SARS-CoV-2 Nucleocapsid protein (SARS-CoV-2 N Products)
- Synonyms
- nucleocapsid phosphoprotein Protein, N Protein
- Target Type
- Viral Protein
- Background
- Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
- Molecular Weight
- 47.4 kDa
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