SARS-CoV-2 Nucleocapsid Protein (SARS-CoV-2 N) (T205I) (His tag)
-
- Target See all SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N) Proteins
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- T205I
-
Origin
- SARS Coronavirus-2 (SARS-CoV-2)
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SARS-CoV-2 Nucleocapsid protein is labelled with His tag.
- Purpose
- SARS-CoV-2 Nucleocapsid protein (T205I), His Tag
- Sequence
- AA 1-419
- Characteristics
- SARS-CoV-2 Nucleocapsid protein (T205I), His Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (T205I). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR)-linker responsible for phosphorylation. The mutation T205I was identified on the nucleocapsid protein of SARS-CoV-2 variants with high frequency in B.1.351 variants circulating in South Africa.
- Purity
- >90 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product SARS-CoV-2 N Protein
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Buffer
- PBS, 0.5 M Arginine, pH 7.4
- Storage
- -20 °C
-
- Target
- SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)
- Alternative Name
- SARS-CoV-2 Nucleocapsid protein (SARS-CoV-2 N Products)
- Synonyms
- nucleocapsid phosphoprotein Protein, N Protein
- Target Type
- Viral Protein
- Background
- Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology, 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
- Molecular Weight
- 47.3 kDa
-