SARS-CoV-2 Spike S1 Protein (RBD) (mFc Tag)
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- Target See all SARS-CoV-2 Spike S1 Proteins
- SARS-CoV-2 Spike S1
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- RBD
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Origin
- SARS Coronavirus-2 (SARS-CoV-2)
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SARS-CoV-2 Spike S1 protein is labelled with mFc Tag.
- Application
- SDS-PAGE (SDS), Functional Studies (Func)
- Sequence
- RVQPTESIVR FPNITNLCPF GEVFNATRFA SVYAWNRKRI SNCVADYSVL YNSASFSTFK CYGVSPTKLN DLCFTNVYAD SFVIRGDEVR QIAPGQTGKI ADYNYKLPDD FTGCVIAWNS NNLDSKVGGN YNYLYRLFRK SNLKPFERDI STEIYQAGST PCNGVEGFNC YFPLQSYGFQ PTNGVGYQPY RVVVLSFELL HAPATVCGPK KSTNLVKNKC VNF
- Specificity
- Arg319-Phe541
- Characteristics
- Recombinant 2019-nCoV Spike RBD Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of 2019-ncov Spike RBD (Accession #YP_009724390.1) fused with a mFc tag at the C-terminus.
- Purity
- > 95 % by SDS-PAGE.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- < 0.1 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 Protein at 2 μg/mL (100 μL/well) can bind Recombinant SARS-COV-2 Spike RBD-mFc Protein, the EC50 of Recombinant SARS-COV-2 Spike RBD-mFc Protein is 2.58 ng/mL.
- Top Product
- Discover our top product SARS-CoV-2 Spike S1 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
- Buffer
- Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.|or This product is stable at ≤ -70°C for up to 6 months from the date of receipt. |For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
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- Target
- SARS-CoV-2 Spike S1
- Abstract
- SARS-CoV-2 Spike S1 Products
- Synonyms
- E2 Protein, Surface Glycoprotein Protein, S Protein
- Target Type
- Viral Protein
- Background
- Envelope,SARS-CoV-2 Spike RBD (N501Y),Spike,Spike ECD,Spike RBD,Spike S1,Spike S2,Spike S2 ECD,S1-RBD protein,NCP-CoV RBD Protein,novel coronavirus RBD Protein,2019-nCoV RBD Protein,S glycoprotein Subunit1 RBD Protein
- Gene ID
- 43740568
- UniProt
- P0DTC2
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