SARS-CoV-2 NSP7, NSP8 protein (His tag)
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- Target
- SARS-CoV-2 NSP7, NSP8
- Protein Type
- Recombinant
- Origin
- SARS Coronavirus-2 (SARS-CoV-2)
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- His tag
- Purpose
- SARS-CoV-2 (COVID-19) NSP7&NSP8 Protein, His Tag
- Sequence
- AA 1-83
- Characteristics
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SARS-CoV-2 NSP7&NSP8, His Tag (NS8-C5125) is expressed from E.coli cells. It contains AA Ser 1 - Gln 83 (NSP7) & Ala 1 - Gln 198 (NSP8) (Accession # YP_009725303.1 (NSP7) & YP_009725304.1 (NSP8). Predicted N-terminus: Met
This protein carries a polyhistidine tag. - Purity
- >95 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- -80 °C
- Storage Comment
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The product MUST be stored at -70°C or lower upon receipt
-70°C for 3 months under sterile conditions.
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- Target
- SARS-CoV-2 NSP7, NSP8
- Alternative Name
- SARS-CoV-2 NSP7 & NSP8
- Target Type
- Viral Protein
- Background
- During the formation of the coronaviral replication/transcription complex, essential steps include processing of the conserved polyprotein nsp7-10 region by the main protease Mpro and subsequent complex formation of the released nsp's. Upon infecting host cells, coronaviruses assemble a multi-subunit RNA-synthesis complex of viral non-structural proteins (nsp) responsible for the replication and transcription of the viral genome. non-structural proteins 7 (NSP7) forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.
- Molecular Weight
- 32.1 kDa
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