SARS-CoV-2 Spike S1 Protein (His tag)

Catalog No. ABIN6952427

Product Details

Target: SARS-CoV-2 Spike S1

Protein Type: Recombinant

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purification tag / Conjugate: This SARS-CoV-2 Spike S1 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Functional Studies (Func)

Sequence: AA 16-685

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 76.9 kDa. The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
AA 16-685

Purity: >90 % as determined by SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Biological Activity Comment: Immobilized 2019-nCoV S1 protein, His Tag at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 2-20 ng/mL (QC tested).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: -20°C

References

Fan, Parr, Kang, Gupta: "Point-of-care (POC) SARS-CoV-2 antigen detection using functionalized aerosol jet-printed organic electrochemical transistors (OECTs)." in: Nanoscale, Vol. 15, Issue 11, pp. 5476-5485, (2023) (PubMed).

Lucas, Klein, Sundaram, Liu, Wong, Silva, Mao, Oh, Mohanty, Huang, Tokuyama, Lu, Venkataraman, Park, Israelow, Vogels, Muenker, Chang, Casanovas-Massana, Moore, Zell, Fournier, Wyllie, Campbell, Lee et al.: "Delayed production of neutralizing antibodies correlates with fatal COVID-19. ..." in: Nature medicine, (2021) (PubMed).

Song, Bartley, Chow, Ngo, Jiang, Zamecnik, Dandekar, Loudermilk, Dai, Liu, Sunshine, Liu, Wu, Hawes, Alvarenga, Huynh, McAlpine, Rahman, Geng, Chiarella, Goldman-Israelow, Vogels, Grubaugh et al.: "Divergent and self-reactive immune responses in the CNS of COVID-19 patients with neurological symptoms. ..." in: Cell reports. Medicine, pp. 100288, (2021) (PubMed).

Maritz, Woudberg, Bennett, Soares, Lapierre, Devine, Kimberg, Bouic: "Validation of high-throughput, semiquantitative solid phase SARS coronavirus-2 serology assays in serum and dried blood spot matrices." in: Bioanalysis, (2021) (PubMed).

Mazzini, Martinuzzi, Hyseni, Benincasa, Molesti, Casa, Lapini, Piu, Trombetta, Marchi, Razzano, Manenti, Montomoli: "Comparative analyses of SARS-CoV-2 binding (IgG, IgM, IgA) and neutralizing antibodies from human serum samples." in: Journal of immunological methods, Vol. 489, pp. 112937, (2021) (PubMed).

Haslwanter, Dieterle, Wec, OBrien, Sakharkar, Florez, Tong, Rappazzo, Lasso, Vergnolle, Wirchnianski, Bortz, Laudermilch, Fels, Mengotto, Malonis, Georgiev, Quiroz, Wrapp, Wang, Dye, Barnhill, Dye et al.: "A Combination of Receptor-Binding Domain and N-Terminal Domain Neutralizing Antibodies Limits the Generation of SARS-CoV-2 Spike Neutralization-Escape Mutants. ..." in: mBio, Vol. 12, Issue 5, pp. e0247321, (2021) (PubMed).

Pierce, Preston-Hurlburt, Dai, Aschner, Cheshenko, Galen, Garforth, Herrera, Jangra, Morano, Orner, Sy, Chandran, Dziura, Almo, Ring, Keller, Herold, Herold: "Immune responses to SARS-CoV-2 infection in hospitalized pediatric and adult patients." in: Science translational medicine, Vol. 12, Issue 564, (2020) (PubMed).

Luetkens, Metcalf, Planelles, Zheng, Larragoite, Spivak, Spivak, Steinbach, Blaylock, Avila, Hankey, Martins, Slev, Mannuel, Sajadi, Rapoport, Atanackovic: "Successful transfer of anti-SARS-CoV-2 immunity using convalescent plasma in an MM patient with hypogammaglobulinemia and COVID-19." in: Blood advances, Vol. 4, Issue 19, pp. 4864-4868, (2020) (PubMed).

Wei, Wan, Yan, Wang, Zhang, Yang, Zhang, Fan, Li, Deng, Sun, Gong, Yang, Wang, Wang, Li, Yang, Li, Zhang, Wang, Du, Zong, Yin, Zhang, Wang, Peng, Lin, Feng, Qin, Chen, Gao, Zhang, Cao, Zhong: "HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry." in: Nature metabolism, Vol. 2, Issue 12, pp. 1391-1400, (2020) (PubMed).

Target Details

Target: SARS-CoV-2 Spike S1

Abstract: SARS-CoV-2 Spike S1 Products

Synonyms: E2 Protein, Surface Glycoprotein Protein, S Protein

Target Type: Viral Protein

Background: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Molecular Weight: 76.9 kDa

Gene ID: 43740568

UniProt: P0DTC2