CXCL12 Protein (AA 24-89) (Biotin)
-
- Target See all CXCL12 Proteins
- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 24-89
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CXCL12 protein is labelled with Biotin.
- Application
- Flow Cytometry (FACS), Cell Migration Assay (CMA)
- Sequence
- KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK
- Characteristics
- Biotininylated Recombinant Human CXCL12 (SDF-1alpha)
- Purity
- > 97 % by SDS PAGE
- Endotoxin Level
- <0.01 EU per 1μg of the protein by the LAL method
- Biological Activity Comment
-
EC50 = 0.5-0.9 nM determined by Calcium Flux with human CXCR4 in U937 cells
Migration confirmed with U937 cells expressing CXCR4 - Top Product
- Discover our top product CXCL12 Protein
-
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 23-89) (Active) protein (His tag,GST tag)
CXCL12 Origin: Human Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 22-89) protein (His tag)CXCL12 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 22-89) protein (His tag)CXCL12 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 22-93) protein (His tag)CXCL12 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) (AA 1-89) protein (GST tag)CXCL12 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Chemokine (C-X-C Motif) Ligand 12 (CXCL12) proteinCXCL12 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 97,00% SDS
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Spin tube prior to resuspending. Recommended at 100 μg/mL in sterile water
- Storage
- -20 °C,-80 °C
- Storage Comment
-
12 months from date of receipt, -20 to -70 °C as supplied.
Suggest to use immediately after reconstitution
1 month at -20 to -70 °C under sterile conditions after reconstitution. - Expiry Date
- 12 months
-
- Target
- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
- Alternative Name
- CXCL12/SDF-1alpha (CXCL12 Products)
- Background
- Stromal-Cell Derived Factor-1alpha (SDF-1alpha) (CXCL12) attracts lymphocytes and plays important roles in embryogenesis and angiogenesis with implications in tumor metastasis. By binding to CXCR4, one of the co-receptors for HIV viral entry, SDF-1alpha can suppress HIV. Besides CXCR4, SDF-1alpha also binds to the “decoy” receptor CXCR7, and activates the downstream beta-arrestin- rather than G protein-mediated signaling pathway.
- Molecular Weight
- 10.381 kDa
- UniProt
- P48061
- Pathways
- Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling
-
