CCL4 Protein (AA 24-92) (Biotin)

Catalog No. ABIN6952193

Product Details

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 24-92

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CCL4 protein is labelled with Biotin.

Application: Cell Migration Assay (CMA)

Sequence: APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN

Characteristics: Biotininylated Recombinant Human CCL4 (MIP-1beta)

Purity: > 97 % by SDS PAGE

Endotoxin Level: <0.01 EU per 1μg of the protein by the LAL method

Biological Activity Comment: EC50 = 0.32-1.3 nM determined by Migration Assay in cells expressing recombinant CCR5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Spin tube prior to resuspending. Recommended at 100 μg/mL in sterile water

Storage: -20 °C,-80 °C

Storage Comment: 12 months from date of receipt, -20 to -70 °C as supplied.
Suggest to use immediately after reconstitution
1 month at -20 to -70 °C under sterile conditions after reconstitution.

Expiry Date: 12 months

Target Details

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Alternative Name: CCL4 (MIP-1beta) (CCL4 Products)

Background: Monokine with inflammatory and chemokinetic properties binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta (3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.

Molecular Weight: 10.238 kDa

UniProt: P13236