tau Protein (partial, Pro301Leu-Mutant)
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- Target See all tau Proteins
- tau (tau Protein)
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Pro301Leu-Mutant, partial
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS), Western Blotting (WB), In vitro Assay (in vitro), In vivo Studies (in vivo)
- Purpose
- Active Human Recombinant Tau (K18), P301L mutant Protein Pre-formed Fibrils
- Sequence
- SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHV LGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE
- Specificity
- ~15.1 kDa
- Purification
- Ion-exchange Purified
- Biological Activity Comment
- Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when active tau PFFs are combined with active tau monomers.
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tau Protein (full length), (Pro301Ser-Mutant) (Active) protein
Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS, WB, in vitro, in vivo Active
tau Protein (AA 241-380) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
tau Protein (AA 200-380) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
tau Protein (AA 1-383) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
tau Protein (AA 1-352) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS - PAGE SDS
tau Protein (AA 1-381) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS - PAGE SDS
tau Protein (AA 1-412) protein (His tag)Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % by SDS - PAGE SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- Lot specific
- Buffer
- 10 mM HEPES, 100 mM NaCl pH 7.4
- Storage
- -80 °C
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- Target
- tau (tau Protein)
- Alternative Name
- Tau (tau Products)
- Synonyms
- DDPAC Protein, FTDP-17 Protein, MAPTL Protein, MSTD Protein, MTBT1 Protein, MTBT2 Protein, PPND Protein, TAU Protein, tau Protein, PHF-tau Protein, microtubule associated protein tau Protein, MAPT Protein
- Background
- Alzheimer's Disease (AD) is the most common neurodegenerative disease, affecting 10 % of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). K18 is a truncated form of human tau containing only the 4 microtubule binding repeats (5). P301L (PL) is a mutation where proline is replaced by leucine at codon 301 of tau, and has been linked to frontotemporal dementia (6).
- UniProt
- P10636
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