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FGF2 Protein

Rockland 009-001-u82-0010 FGF2 Origin: Human Host: Escherichia coli (E. coli) Recombinant 97,00% SDS
Rockland
Catalog No. ABIN6700913
Supplier Product No.: 009-001-u82-0010
  • Target See all FGF2 Proteins
    FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
    Protein Type
    Recombinant
    Origin
    • 25
    • 11
    • 9
    • 6
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 48
    • 9
    • 1
    • 1
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Supplier Product No.
    009-001-u82-0010
    Supplier
    Rockland
    Purpose
    Human Fibroblast Growth Factor 147 basic Recombinant Protein
    Purification
    Fibroblast Growth Factor 147 basic purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
    Purity
    97,00%
    Endotoxin Level
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells and is typically less than 1 ng/mL.
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    Discover our top product FGF2 Protein
  • Application Notes

    Other: User Optimized

    Application_Note: Fibroblast Growth Factor 147 basic Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Fibroblast Growth Factor 147 basic in immunological assays.

    Comment

    Suggested_Applications: Cellular Assay

    Other_Performance_Data:

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 10 μL (10-100 μL)

    Buffer
    Lyophilized in 10 mM sodium phosphate, 75 mM sodium chloride, pH 7.5.
    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Expiry Date
    6 months
  • Target
    FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))
    Alternative Name
    FGF2 (FGF2 Products)
    Background

    Synonyms: Heparin-binding growth factor 2 (HBGF-2), Prostatropin, Basic fibroblast growth factor (bFGF)

    Background: Fibroblast Growth Factors (FGFs) are a 22 member family of proteins known to be involved in angiogenesis, wound healing and embryonic development. As a family, they bind to heparin and signal through four receptor tyrosine kinases called, FGFR1, 2, 3 and 4. Although the mechanism remains unclear, FGF-basic 147 (variant of FGF basic 154), also called FGF-2, is a critical component in keeping embryonic stem cells undifferentiated in cell culture systems. Recombinant human FGF-b 147 (FGF-2) is a non-glycosylated protein, containing 147 amino acids, with a molecular weight of 16.5 kDa.

    UniProt
    P09038
    Pathways
    RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins
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