CCL4 Protein

Catalog No. ABIN6700901

Supplier Product No.: 010-001-b85

Product Details

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 010-001-b85

Supplier: Rockland

Purpose: MIP-1ß Mouse Recombinant Protein

Purification: Purity was determined to be greater than 95% as determined by analysis by RP-HPLC and by reducing and non- reducing SDS-PAGE.

Purity: 95,00%

Endotoxin Level: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: Mouse Macrophage Inflammatory Protein-1 beta is fully biologically active when compared to standard. The activity is calculated by the ability to chemoattract blood monocytes at 20-100 ng/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: MIP-1ß protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-MIP-1ß in immunological assays.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Alternative Name: Ccl4 (CCL4 Products)

Background:

Synonyms: Mouse Macrophage Inflammatory Protein-1-beta (CCL4), C-C motif chemokine 4, Small-inducible cytokine A4, Macrophage inflammatory protein 1-beta, MIP-1-beta, Immune activation protein 2, ACT-2, ACT2, Protein H400, SIS-gamma, Mip1b, Scya4

Background: MIP-1ß is a secreted monokine with inflammatory and chemokinetic properties and can exist as a homodimer. It belongs to the intercrine beta (chemokine CC) family. Recombinant Mouse Macrophage Inflammatory Protein-1 beta produced in E. coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7,808 Daltons.

UniProt: P14097