IL17F Protein

Catalog No. ABIN6700895

Supplier Product No.: 012-001-b31

Product Details

Target: IL17F (Interleukin 17F (IL17F))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 012-001-b31

Supplier: Rockland

Purpose: IL-17F Rat Recombinant Protein

Purification: Purity was determined to be greater than 98% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.

Purity: 98,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: IL-17F activity is determined by the dose-dependent induction of IL-6 production in cultured mouse NIH 3T3 fibroblasts and is typically 10-20 ng/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: IL-17F protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-17F in immunological assays.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 25 μL (25-250 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: IL17F (Interleukin 17F (IL17F))

Alternative Name: Il17f (IL17F Products)

Background:

Synonyms: Interleukin-17F cytokine, IL-17F, Interleukin-24, IL-24, Cytokine ML-1

Background: Recombinant Rat IL-17F produced in E.coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 133 amino acids and having a molecular mass of 14.9 kDa. Homodimeric interleukin-17F is a member of the IL-17 family of six homodimeric proteins (IL-17A-17F). Activity studies reveal IL-17A to be the most potent, followed by IL-17A/F and IL-17F 100 fold lower than IL-17A.

UniProt: Q5BJ95

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity