YWHAE Protein
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- Target See all YWHAE Proteins
- YWHAE (14-3-3 epsilon (YWHAE))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r26s
- Supplier
- Rockland
- Purpose
- 14-3-3 epsilon recombinant protein
- Purification
- Recombinant full-length human tag-free 14-3-3ε was expressed in E. coli cells. The purity was determined to be >90% by densitometry.
- Purity
- >90%
- Top Product
- Discover our top product YWHAE Protein
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- Application Notes
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Western_Blot_Dilution: User Optimized
Application_Note: 14-3-3ε Protein is suitable for use in Western Blot. Expect a band approximately ~30 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 μg/μL
- Buffer
- 14-3-3ε Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- YWHAE (14-3-3 epsilon (YWHAE))
- Alternative Name
- YWHAE (YWHAE Products)
- Background
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Synonyms: 14-3-3 epsilon, YWHAE, MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465
Background: 14-3-3ε also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein is a member of the 14-3-3 family. 14-3-3ε has been reported to be involved in heat shock transcription factor 1 (HSF1) and extracellular signal regulated protein kinase (ERK) pathways (1). 14-3-3ε interaction with the deubiquitinating enzyme UBPY, also known as USP8, regulates cargo sorting and membrane traffic at early endosomes. Association of 14-3-3ε, HSF1 with ERK during heat shock may modulate the amplitude of the response and control the termination of HSP expression on resumption of growth conditions (2). 14-3-3ε protein is ideal for investigators involved in Cell Stress & Chaperone Proteins, Cell Signaling, Cancer research, Cell Cycle, Cellular Stress, Neurobiology, and WNT Signaling research.
- NCBI Accession
- NM_006761
- Pathways
- Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase
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