TRAIL Protein
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- Target See all TRAIL (TNFSF10) Proteins
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-w34-0050
- Supplier
- Rockland
- Purpose
- Human TRAIL Recombinant Protein
- Purification
- TRAIL purity was determined to be greater than 90% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
- Purity
- 90,00%
- Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the ability to induce apoptotic cell death in TRAIL-sensitive RPMI-8226 cells and is typically 10-15 ng/mL.
- Top Product
- Discover our top product TNFSF10 Protein
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- Application Notes
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Other: User Optimized
Application_Note: TRAIL Recombinant Protein ha been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-TRAIL in immunological assays.
- Comment
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 50μL
- Buffer
- Product is provided as lyophilized powder in 0.05M Sodium Chloride and 0.01M Sodium Phosphate, pH 7.5.
- Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
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- Target
- TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
- Alternative Name
- TNFSF10 (TNFSF10 Products)
- Background
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Synonyms: Apo2 Ligand (Apo2L), TL2, TNF-related apoptosis-inducing ligand, CD253
Background: TNF-Related Apoptosis-Inducing Ligand (TRAIL) is produced by a wide variety of cell types and is shown to be a cytotoxic protein that induces apoptosis in tumor cells through activation of the death receptors, DR4 and DR5. Human TRAIL is active on mouse cells. Recombinant human TRAIL is a non-glycosylated protein, containing the 168 amino acid extracellular portion of TRAIL that is homologous to TNF. It has a molecular weight of 19.5 kDa.
- UniProt
- P50591
- Pathways
- Apoptosis, Positive Regulation of Endopeptidase Activity
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