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TRAIL Protein

Rockland 009-001-w34-0010 TNFSF10 Origin: Human Host: Escherichia coli (E. coli) Recombinant 90,00% SDS
Rockland
Catalog No. ABIN6700756
Supplier Product No.: 009-001-w34-0010
  • Target See all TRAIL (TNFSF10) Proteins
    TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
    Protein Type
    Recombinant
    Origin
    • 23
    • 8
    • 2
    • 1
    Human
    Source
    • 23
    • 3
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Supplier Product No.
    009-001-w34-0010
    Supplier
    Rockland
    Purpose
    Human TRAIL Recombinant Protein
    Purification
    TRAIL purity was determined to be greater than 90% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
    Purity
    90,00%
    Endotoxin Level
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is determined by the ability to induce apoptotic cell death in TRAIL-sensitive RPMI-8226 cells and is typically 10-15 ng/mL.
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    Discover our top product TNFSF10 Protein
  • Application Notes

    Other: User Optimized

    Application_Note: TRAIL Recombinant Protein ha been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-TRAIL in immunological assays.

    Comment

    Suggested_Applications: Cellular Assay

    Other_Performance_Data:

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 10 μL (10-100 μL)

    Buffer
    Product is provided as lyophilized powder in 0.05M Sodium Chloride and 0.01M Sodium Phosphate, pH 7.5.
    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Expiry Date
    6 months
  • Target
    TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))
    Alternative Name
    TNFSF10 (TNFSF10 Products)
    Background

    Synonyms: Apo2 Ligand (Apo2L), TL2, TNF-related apoptosis-inducing ligand, CD253

    Background: TNF-Related Apoptosis-Inducing Ligand (TRAIL) is produced by a wide variety of cell types and is shown to be a cytotoxic protein that induces apoptosis in tumor cells through activation of the death receptors, DR4 and DR5. Human TRAIL is active on mouse cells. Recombinant human TRAIL is a non-glycosylated protein, containing the 168 amino acid extracellular portion of TRAIL that is homologous to TNF. It has a molecular weight of 19.5 kDa.

    UniProt
    P50591
    Pathways
    Apoptosis, Positive Regulation of Endopeptidase Activity
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