Thrombopoietin Protein (THPO)

Catalog No. ABIN6700706

Supplier Product No.: 010-001-w32-0100

Product Details

Target: Thrombopoietin (THPO)

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 010-001-w32-0100

Supplier: Rockland

Purpose: Mouse Thrombopoietin Recombinant Protein

Purification: Thrombopoietin purity was determined to be greater than 97% as determined by reducing and non-reducing SDS-pAGE.

Purity: 97,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the dose-dependent stimulation of MO7e cells and is typically less than 1 ng/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: Thrombopoietin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Thrombopoietin in immunological assays.

Comment:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, pH 7.5

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: Thrombopoietin (THPO)

Alternative Name: Thpo (THPO Products)

Background:

Synonyms: C-mpl ligand (ML), Megakaryocyte Colony Stimulating Factor, Megakaryocyte growth and development factor (MGDF), Myeloproliferative leukemia virus oncogene ligan, c-MPL ligand

Background: Thrombopoietin (TPO) is a growth factor that is produced by the liver and kidney. TPO acts through the TPO receptor to promote megakaryocyte maturation and differentiation, which leads to the production of platelets. Recombinant mouse TPO is a non-glycosylated protein, containing 174 amino acids (which comprise the receptor binding domain), with a molecular weight of 18.7 kDa.

UniProt: P40226

Pathways: JAK-STAT Signaling, Hormone Activity