CXCL12 Protein

Catalog No. ABIN6700579

Supplier Product No.: 010-001-w23-0010

Product Details

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 010-001-w23-0010

Supplier: Rockland

Purpose: Mouse Stromal Cell-Derived Factor-1 beta (CXCL12) Recombinant Protein

Purification: Stromal Cell-Derived Factor-1 beta (CXCL12) purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.

Purity: 97,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to chemoattract human T cells at 10-75 ng/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: Stromal Cell-Derived Factor-1 beta Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Stromal Cell-Derived Factor-1 beta in immunological assays.

Comment:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))

Alternative Name: Cxcl12 (CXCL12 Products)

Background:

Synonyms: 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 (TPAR1), C-X-C motif chemokine 12, Pre-B cell growth-stimulating factor (PBSF), Thymic lymphoma cell-stimulating factor (TLSF)

Background: Stromal Derived Growth Factor-1β (SDF-1β), also called CXCL12, is one of two splice variants made by a wide variety of cells when stimulated by inflammatory cytokines such as, TNF, IL-1 or LPS. SDF-1β signals through the G protein-couple receptor, CXCR4, to recruit activated leukocytes. Human SDF-1β shares 100 % identity to feline SDF-1β. Recombinant mouse SDF-1β is a non-glycosylated protein, containing 72 amino acids, with a molecular weight of 8.5 kDa.

UniProt: Q4FJL5

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling