Stathmin 1 Protein (STMN1) (His tag)

Catalog No. ABIN6700567

Supplier Product No.: 009-001-t58s

Product Details

Target: Stathmin 1 (STMN1)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Stathmin 1 protein is labelled with His tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-t58s

Supplier: Rockland

Purpose: Stathmin recombinant protein-HIS Epitope

Purification: Recombinant full-length human Stathmin protein was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >90% by densitometry.

Purity: >90%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Application_Note: Stathmin Protein is suitable for use in Western Blot. Expect a band approximately ~19 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: Stathmin Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Stathmin 1 (STMN1)

Alternative Name: STMN1 (STMN1 Products)

Background:

Synonyms: STMN1, C1orf215, FLJ32206, Lag, LAP18, MGC138869, MGC138870, OP18, PP17, PP19, PR22, SMN, Stathmin

Background: Stathmin is a ubiquitous, highly conserved protein present in the cytoplasm in a variety of unphosphorylated and phosphorylated forms. Stathmin functions as an intracellular relay integrating regulatory signals of the cellular environment (1). Stathmin is involved in the regulation of the microtubule filament system. Stathmin binds tubulin in a ternary complex, with 2 tubulins for every stathmin and this complex interferes with microtubule dynamics in vitro and in vivo. Overexpression of several stathmin mutants suggest that stathmin induces depolymerization of interphase and mitotic microtubules in its unphosphorylated state, but is inactivated by phosphorylation in mitosis (2). Stathmin Protein is ideal for investigators involved in Signaling Proteins, Microtubule/Actin Associated Proteins, Cancer, Invasion/Metastasis, and Neurobiology research.

Pathways: MAPK Signaling, Microtubule Dynamics