Sonic Hedgehog Protein (SHH)
-
- Target See all Sonic Hedgehog (SHH) Proteins
- Sonic Hedgehog (SHH)
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-w24-0005
- Supplier
- Rockland
- Purpose
- Human Sonic Hedgehog Recombinant Protein
- Purification
- Sonic Hedgehog purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
- Purity
- 97,00%
- Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 (CCL-226) fibroblasts and is typically 0.8-1 μg/mL.
- Top Product
- Discover our top product SHH Protein
-
-
- Application Notes
-
Other: User Optimized
Application_Note: Sonic Hedgehog Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Sonic Hedgehog in immunological assays.
- Comment
-
Suggested_Applications: Cellular Assay
Other_Performance_Data:
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 5 μL (5-50 μL)
- Buffer
-
Buffer: 0.01 M Sodium Phosphate, pH 7.5
Stabilizer: None
- Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
-
- Target
- Sonic Hedgehog (SHH)
- Alternative Name
- SHH (SHH Products)
- Background
-
Synonyms: HHG-1
Background: Sonic hedgehog (SHH) is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60 % homology and all signal via the same receptors. Recombinant human SHH is a non-glycosylated protein, containing 175 amino acids, with a molecular weight of 19.7 kDa. The Cys at position 25 has been substituted with Ile.
- UniProt
- Q15465
- Pathways
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
-