Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

SIRT1 Protein (GST tag)

Rockland 009-001-t31s SIRT1 Origin: Human Host: Escherichia coli (E. coli) Recombinant >80% WB
Rockland
Catalog No. ABIN6700527
Supplier Product No.: 009-001-t31s
  • Target See all SIRT1 Proteins
    SIRT1 (Sirtuin 1 (SIRT1))
    Protein Type
    Recombinant
    Origin
    • 7
    • 3
    • 2
    • 1
    • 1
    Human
    Source
    • 8
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This SIRT1 protein is labelled with GST tag.
    Application
    Western Blotting (WB)
    Supplier Product No.
    009-001-t31s
    Supplier
    Rockland
    Purpose
    SIRT1 recombinant protein-GST fusion protein
    Purification
    Recombinant human SIRT1 (193-end) was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.
    Purity
    >80%
    Top Product
    Discover our top product SIRT1 Protein
  • Application Notes

    Western_Blot_Dilution: User Optimized

    Application_Note: SIRT1 Protein is suitable for use in Western Blot. Expect a band approximately ~105 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 μg/μL
    Buffer
    SIRT1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    SIRT1 (Sirtuin 1 (SIRT1))
    Alternative Name
    SIRT1 (SIRT1 Products)
    Background

    Synonyms: SIR2L1, Sirtuin 1,NAD-dependent protein deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, SirtT1 75 kDa fragment, 75SirT1

    Background: SIRT1 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT1 being a member of class I. SIRT1 is a stress-response and chromatin-silencing factor (1). It is an NAD(+)-dependent histone deacetylase involved in various nuclear events such as transcription, DNA replication, and DNA repair. SIRT1 protein binds and deacetylates the p53 protein (2). Expression of wild type SIRT1 in human cells reduces the transcriptional activity of p53 indicating that SIRT1 is involved in the regulation of p53 function via deacetylation. SIRT1 Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.

    NCBI Accession
    NM_012238
    Pathways
    MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling
You are here:
Support