Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

SCYL3 Protein (GST tag)

Rockland 009-001-t27s SCYL3 Origin: Human Host: Insect cells (Sf9) Recombinant >85% WB
Rockland
Catalog No. ABIN6700491
Supplier Product No.: 009-001-t27s
  • Target See all SCYL3 Proteins
    SCYL3 (SCY1-Like 3 (SCYL3))
    Protein Type
    Recombinant
    Origin
    Human
    Source
    • 1
    • 1
    Insect cells (Sf9)
    Purification tag / Conjugate
    This SCYL3 protein is labelled with GST tag.
    Application
    Western Blotting (WB)
    Supplier Product No.
    009-001-t27s
    Supplier
    Rockland
    Purpose
    SCYL3 recombinant protein-GST fusion protein
    Purification
    Recombinant human SCYL3 (2-end) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.
    Purity
    >85%
    Top Product
    Discover our top product SCYL3 Protein
  • Application Notes

    Western_Blot_Dilution: User Optimized

    Application_Note: SCYL3 Protein is suitable for use in Western Blot. Expect a band approximately ~110 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.1 μg/μL
    Buffer
    SCYL3 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    Storage
    -80 °C
    Storage Comment
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    SCYL3 (SCY1-Like 3 (SCYL3))
    Alternative Name
    SCYL3 (SCYL3 Products)
    Background

    Synonyms: PACE-1, PACE1, RP1-97P20.2, Protein-associating with the carboxyl-terminal domain of ezrin, Ezrin-binding protein PACE-1, SCY1-like protein 3, SCYL3

    Background: SCYL3 encodes a protein with a kinase domain and four HEAT repeats which interact with the C-terminal domain of ezrin, an ERM protein which play a role in cell adhesion and migration (1). C-terminal domain of SCYL3 interacts with the C-terminal alpha-helical domain of ezrin. Biochemical assays indicate that SCYL3 had associated rather than intrinsic protein kinase activity. SCYL3 is associated with the cytoplasmic face of the Golgi apparatus. This distribution is dependent upon the presence of the SCYL3 N-terminal myristoylation consensus sequence but is not dependent on an association with ezrin. SCYL3 is highly expressed in breast carcinoma cells and adult colon. SCYL3 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Inflammation, Invasion/Metastasis, and Neurobiology research.

You are here:
Support