SCYL1 Protein (GST tag)
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- Target See all SCYL1 Proteins
- SCYL1 (SCY1-Like 1 (SCYL1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Insect cells (Sf9)
- Purification tag / Conjugate
- This SCYL1 protein is labelled with GST tag.
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-t26s
- Supplier
- Rockland
- Purpose
- SCYL1 recombinant protein-GST fusion protein
- Purification
- Recombinant human SCYL1 (1-556) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.
- Purity
- >95%
- Top Product
- Discover our top product SCYL1 Protein
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- Application Notes
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Western_Blot_Dilution: User Optimized
Application_Note: SCYL1 Protein is suitable for use in Western Blot. Expect a band approximately ~85 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.1 μg/μL
- Buffer
- SCYL1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- SCYL1 (SCY1-Like 1 (SCYL1))
- Alternative Name
- SCYL1 (SCYL1 Products)
- Background
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Synonyms: GKLP, HT019, NKTL, NTKL, P105, TAPK, TEIF, TRAP, N-terminal kinase-like protein, Coated vesicle-associated kinase of 90 kDa, SCY1-like protein 1, Telomerase regulation-associated protein, Telomerase transcriptional element-interacting factor, Teratoma-associated tyrosine kinase
Background: SCYL1 is a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. SCYL1 has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. SCYL1 activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. SCYL1 forms multimers following transfection into COS-7 cells (1). A SCYL1 binding protein has been identified that co-localized with SCYL1 in cytoplasm and shows ubiquitous expression (2). SCYL1 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Inflammation, Invasion/Metastasis, and Neurobiology research.
- NCBI Accession
- NM_020680
- Pathways
- p53 Signaling
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