Retinoblastoma 1 Protein (RB1) (GST tag)

Catalog No. ABIN6700477

Supplier Product No.: 009-001-t16s

Product Details

Target: Retinoblastoma 1 (RB1)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Retinoblastoma 1 protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-t16s

Supplier: Rockland

Purpose: Rb recombinant protein-GST fusion protein

Purification: Recombinant human Rb (773-928) was expressed in E.coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.

Purity: >85%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Other: Kinase Assay-User Optimized

Application_Note: Rb Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~44 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: Rb Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Retinoblastoma 1 (RB1)

Alternative Name: RB1 (RB1 Products)

Background:

Synonyms: RB1, OSRC, pRb, pp110, p105-Rb, Retinoblastoma 1

Background: Rb is the retinoblastoma gene product and was the first tumor suppressor cloned. Rb is a negative regulator of the cell cycle through its ability to bind the transcription factor E2F and repress transcription of genes required for S phase (1). Rb can bind to MDM2 and this overcomes both the antiapoptotic function of MDM2 and the MDM2-dependent degradation of p53. Rb associates with the Polycomb group (PcG) proteins to form a repressor complex that blocks entry of cells into mitosis (2). Rb colocalizes with nuclear PcG complexes and is important for association of PcG complexes with nuclear targets. Rb Protein is ideal for investigators involved in Signaling Reagents, Protein Substrates, Cancer, Cell Cycle, and Neurobiology research.

NCBI Accession: NM_000321

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy