RAS Protein (GST tag)
Origin: Human
Host: Escherichia coli (E. coli)
WB
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- Target See all RAS products
- RAS
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RAS protein is labelled with GST tag.
- Application
- Western Blotting (WB)
- Cross-Reactivity
- Human
- Characteristics
- K-ras, N-ras, RASH1, c-bas/has, Harvey rat sarcoma viral oncogene homolog
- Purification
- Recombinant full length human RAS was expressed in E.coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.
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- Application Notes
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Application Note: RAS Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. RAS Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~47 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.2 μg/μL
- Storage
- -80 °C
- Storage Comment
- -80 °c
- Expiry Date
- 12 months
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- Target
- RAS
- Abstract
- RAS Products
- Synonyms
- asr Protein, resistance to audiogenic seizures Protein, ras Protein
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