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PDGFB Protein

Rockland 010-001-w12-0010 PDGFB Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 98,00%
Rockland
Catalog No. ABIN6700408
Supplier Product No.: 010-001-w12-0010
  • Target See all PDGFB Proteins
    PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
    Protein Type
    Recombinant
    Origin
    • 11
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Mouse
    Source
    • 18
    • 3
    • 1
    Escherichia coli (E. coli)
    Supplier Product No.
    010-001-w12-0010
    Supplier
    Rockland
    Purpose
    Mouse Platelet Derived Growth Factor BB Recombinant Protein
    Purification
    platelet Derived Growth Factor BB purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
    Purity
    98,00%
    Endotoxin Level
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is measured by the dose-dependent proliferation in mouse 3T3 fibroblasts and is typically 1-2 ng/mL.
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    Discover our top product PDGFB Protein
  • Application Notes

    Other: User Optimized

    Application_Note: Platelet Derived Growth Factor-BB Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-BB in immunological assays.

    Comment

    Suggested_Applications: Cellular Assay

    Other_Performance_Data:

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 10 μL (10-100 μL)

    Concentration
    0.1 mg/mL
    Buffer

    Buffer: 0.01 M Sodium Citrate, pH 3.0

    Stabilizer: None

    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Expiry Date
    6 months
  • Target
    PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
    Alternative Name
    Pdgfb (PDGFB Products)
    Background

    Synonyms: PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis

    Background: Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. Recombinant mouse PDGF-BB is a non-glycosylated, disulfide-linked homodimer, containing two 109 amino acids chains, with a total molecular weight of 24.6 kDa.

    UniProt
    P31240
    Pathways
    RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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