PDGFB Protein
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- Target See all PDGFB Proteins
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-w12-0100
- Supplier
- Rockland
- Purpose
- Human Platelet Derived Growth Factor-BB Recombinant Protein
- Purification
- platelet Derived Growth Factor-BB purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
- Purity
- 95,00%
- Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of 3T3 indicator cells and is typically 1-3 ng/mL.
- Top Product
- Discover our top product PDGFB Protein
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- Application Notes
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Other: User Optimized
Application_Note: Platelet Derived Growth Factor-BB Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-BB in immunological assays.
- Comment
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
- Concentration
- 0.1 mg/mL
- Buffer
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Buffer: 0.01 M Sodium Phosphate, pH 7.5
Stabilizer: None
- Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
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- Target
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
- Alternative Name
- PDGFB (PDGFB Products)
- Background
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Synonyms: PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis
Background: Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. Recombinant human PDGF-BB is a non-glycosylated, disulfide-linked homodimer, containing two 109 amino acid chains, with a total molecular weight of 24.3 kDa.
- UniProt
- P01127
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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