PDGFA Protein
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- Target See all PDGFA Proteins
- PDGFA (Platelet Derived Growth Factor A (PDGFA))
- Protein Type
- Recombinant
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 010-001-w08-0010
- Supplier
- Rockland
- Purpose
- Mouse Platelet Derived Growth Factor AA Recombinant Protein
- Purification
- platelet Derived Growth Factor AA purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
- Purity
- 97,00%
- Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of Balb/c 3T3 cells and is typically 8-10 ng/mL.
- Top Product
- Discover our top product PDGFA Protein
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Platelet Derived Growth Factor A (PDGFA) (Active) protein
PDGFA Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Platelet Derived Growth Factor A (PDGFA) (AA 1-211) protein (GST tag)PDGFA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Platelet Derived Growth Factor A (PDGFA) (AA 94-194) (Active) protein (His tag)PDGFA Origin: Mouse Host: Escherichia coli (E. coli) > 95 % AcA, CC Active
Platelet Derived Growth Factor A (PDGFA) (Transcript Variant 2) (Active) proteinPDGFA Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, STD, PI Active
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- Application Notes
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Other: User Optimized
Application_Note: Platelet Derived Growth Factor-AA Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-AA in immunological assays.
- Comment
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- Buffer
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
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- Target
- PDGFA (Platelet Derived Growth Factor A (PDGFA))
- Alternative Name
- Pdgfa (PDGFA Products)
- Background
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Synonyms: PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide
Background: Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. Recombinant mouse PDGF-AA is a non-glycosylated, disulfide-linked homodimer, containing two 126 amino acid chains, with a total molecular weight of 28.9 kDa.
- UniProt
- P20033
- Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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