PDGF Protein
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- Target
- PDGF
- Protein Type
- Recombinant
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-w10-0010
- Supplier
- Rockland
- Purpose
- Human Platelet Derived Growth Factor-AB Recombinant Protein
- Purification
- platelet Derived Growth Factor-AB purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
- Purity
- 95,00%
- Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically less than 1 ng/mL.
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- Application Notes
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Other: User Optimized
Application_Note: Platelet Derived Growth Factor-AB Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-AB in immunological assays.
- Comment
-
Suggested_Applications: Cellular Assay
Other_Performance_Data:
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- Concentration
- 0.1 mg/mL
- Buffer
-
Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- Preservative
- Without preservative
- Storage
- -20 °C
- Storage Comment
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
-
- Target
- PDGF
- Alternative Name
- PDGFA/PDGFB
- Background
-
Synonyms: PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, proto-oncogene c-Sis
Background: Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. Recombinant human PDGF-AB is a non-glycosylated, disulfide-linked heterodimer, containing one 14.3 kDa alpha-chain and one 12.1 kDa beta-chain, with total molecular weight of 26.4 kDa.
- UniProt
- P04085
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