MGMT Protein (His tag)

Catalog No. ABIN6700327

Product Details

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Origin: Human

Source: Baculovirus infected Insect Cells, Insect cells (Sf9)

Purification tag / Conjugate: This MGMT protein is labelled with His tag.

Application: Western Blotting (WB)

Cross-Reactivity: Human

Characteristics: Methylated-DNA--protein-cysteine methyltransferase, EC 2.1.1.63, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase

Purification: Recombinant full length human MGMT was expressed by baculovirus in Sf9 insect cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.

Application Details

Application Notes:

Application Note: MGMT Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. MGMT Protein is suitable for use in Western Blot. Expect a band approximately ~25 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 μg/μL

Storage: -80 °C

Storage Comment: -80 °c

Expiry Date: 12 months

Target Details

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT (MGMT Products)

Synonyms: AGT Protein, AI267024 Protein, Agat Protein, O-6-methylguanine-DNA methyltransferase Protein, MGMT Protein, Mgmt Protein

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus