NAMPT Protein
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- Target See all NAMPT Proteins
- NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-w40-0100
- Supplier
- Rockland
- Purpose
- Human Visfatin Recombinant Protein
- Purification
- Visfatin purity was determined to be greater than 90% as determined by reducing and non-reducing SDS-pAGE.
- Purity
- 90,00%
- Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by its ability to induce IL-6, IL-1 beta and TNF alpha production from human PBMCs at 100 ng/mL.
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- Discover our top product NAMPT Protein
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- Application Notes
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Other: User Optimized
Application_Note: Visfatin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Visfatin in immunological assays.
- Comment
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution_Buffer: 0.02M HCl
Reconstitution_Volume: 100 μL
- Buffer
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
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- Target
- NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))
- Alternative Name
- NAMPT (NAMPT Products)
- Background
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Synonyms: Pre-B-cell colony-enhancing factor 1, PBEF
Background: Visfatin is an adipokine produced by visceral adipose tissue. Other adipokines include leptin, adiponectin, resistin, chemerin, omentin and vaspin. Visfatin has been shown to act as a pro-inflammatory factor for PBMCs and may mimic the effects of insulin on adipocytes, monocytes and hepatocytes. Recombinant human Visfatin is a non-glycosylated protein, containing 466 amino acids, with a molecular weight of 52.6 kDa.
- UniProt
- P43490
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