NAMPT Protein

Catalog No. ABIN6700314

Supplier Product No.: 009-001-w40-0025

Product Details

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 009-001-w40-0025

Supplier: Rockland

Purpose: Human Visfatin Recombinant Protein

Purification: Visfatin purity was determined to be greater than 90% as determined by reducing and non-reducing SDS-pAGE.

Purity: 90,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by its ability to induce IL-6, IL-1 beta and TNF alpha production from human PBMCs at 100 ng/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: Visfatin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Visfatin in immunological assays.

Comment:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: 0.02M HCl

Reconstitution_Volume: 25 μL (25-250 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: NAMPT (NAMPT Products)

Background:

Synonyms: Pre-B-cell colony-enhancing factor 1, PBEF

Background: Visfatin is an adipokine produced by visceral adipose tissue. Other adipokines include leptin, adiponectin, resistin, chemerin, omentin and vaspin. Visfatin has been shown to act as a pro-inflammatory factor for PBMCs and may mimic the effects of insulin on adipocytes, monocytes and hepatocytes. Recombinant human Visfatin is a non-glycosylated protein, containing 466 amino acids, with a molecular weight of 52.6 kDa.

UniProt: P43490