NGFB Protein
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- Target See all NGFB Proteins
- NGFB (Nerve Growth Factor beta (NGFB))
- Protein Type
- Recombinant
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 010-001-w03-0020
- Supplier
- Rockland
- Purpose
- Mouse Nerve Growth Factor beta Recombinant Protein
- Purification
- Nerve Growth Factor beta purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
- Purity
- 95,00%
- Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity as determined by the proliferation of TF-1 cells and is typically less than 1 ng/mL.
- Top Product
- Discover our top product NGFB Protein
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- Application Notes
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Other: User Optimized
Application_Note: Nerve Growth Factor beta Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Nerve Growth Factor beta in immunological assays.
- Comment
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 20 μL (20-200 μL)
- Buffer
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
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- Target
- NGFB (Nerve Growth Factor beta (NGFB))
- Alternative Name
- NGF beta (NGFB Products)
- Background
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Synonyms: beta-NGF
Background: Nerve Growth Factor (NGF-β) is a neurotrophic factor related to BDNF, NT-3 and NT-4. NGF-β acts through its receptor β-NGFR, and is involved in the development and maintenance of the sensory and sympathetic nervous systems. NGF-β also is also involved in the growth, differentiation and survival of B lymphocytes. Human, mouse and rat proteins show cross-reactivity. Recombinant mouse NGF-β is a non-glycosylated, non-covalently linked homodimer, containing two 120 amino acid chains , with a molecular weight of 13.5 kDa each.
- UniProt
- Q6LDU8
- Pathways
- NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size
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