MSTN Protein

Catalog No. ABIN6700275

Supplier Product No.: 009-001-w01-0010

Product Details

Target: MSTN (Myostatin (MSTN))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 009-001-w01-0010

Supplier: Rockland

Purpose: Human Myostatin Recombinant Protein

Purification: Myostatin purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Purity: 95,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the inhibition of the proliferation of MPC-11 cells and is typically less than 20 ng/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: Myostatin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Myostatin in immunological assays.

Comment:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: 0.02M HCl

Reconstitution_Volume: 10 μL (10-100 μL)

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: MSTN (Myostatin (MSTN))

Alternative Name: Mstn (MSTN Products)

Background:

Synonyms: GDF-8, Myostatin

Background: Myostatin is a member of the TGF-β superfamily that is essential for proper regulation of skeletal muscle mass in a number of species ranging from fish to humans. Myostatin is a secreted protein that negatively regulates skeletal muscle mass determining both muscle fiber number and size. Inhibition of myostatin can increase muscle mass in a number of animal models of human disease, including muscular dystrophy. Recombinant human Myostatin is a non-glycosylated homodimer, containing two 109 amino acid chains, with a total molecular weight of 24.8 kDa.

UniProt: O14793