Moesin Protein (MSN) (GST tag)

Catalog No. ABIN6700268

Supplier Product No.: 009-001-s50

Product Details

Target: Moesin (MSN)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Moesin protein is labelled with GST tag.

Application: Western Blotting (WB)

Supplier Product No.: 009-001-s50

Supplier: Rockland

Purpose: Moesin recombinant protein-GST fusion protein

Purification: Recombinant human Moesin (410-end) was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.

Purity: >95%

Application Details

Application Notes:

Western_Blot_Dilution: User Optimized

Other: Kinase Assay-User Optimized

Application_Note: Moesin Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~50 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: Moesin Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

Storage: -80 °C

Storage Comment: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Expiry Date: 12 months

Target Details

Target: Moesin (MSN)

Alternative Name: MSN (MSN Products)

Background:

Synonyms: MSN, Moesin Membrane-organizing extension spike protein

Background: Moesin (or membrane-organizing extension spike protein) belongs to ERM family that modulates epithelial integrity by regulating cell-signalling events that affect actin organization and polarity (1). The effects of Moesin on epithelial cells appear to result from inhibition of Rho signaling. ERM proteins serve a structural role in linkage of the cytoskeleton to the plasma membrane and the rescue of cells lacking Moesin by modulation of Rho signaling indicates that inhibition of Rho activity may be a more critical function of Moesin. The negative feedback loop produced by Rho's activation of ERM may be an important mechanism that prevents the excessive migratory and invasive properties characteristic of metastatic cancer cells (2). Moesin Protein is ideal for investigators involved in Signaling Reagents, Protein Substrates, Inflammation, and PKA/PKC Pathway research.

NCBI Accession: NM_002444

Pathways: Asymmetric Protein Localization