Galectin 3 Protein (LGALS3)

Catalog No. ABIN6700206

Supplier Product No.: 009-001-u92-0010

Product Details

Target: Galectin 3 (LGALS3)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 009-001-u92-0010

Supplier: Rockland

Purpose: Human Galectin-3 Recombinant Protein

Purification: Galectin-3 purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Purity: 97,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by the ability to induce chemotaxis of human PBMCs at concentrations ranging from 2-220 μg/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: Galectin-3 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Galectin-3 in immunological assays.

Comment:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer: Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: Galectin 3 (LGALS3)

Alternative Name: LGALS3 (LGALS3 Products)

Background:

Synonyms: 35 kDa lectin, Galactose-specific lectin-3, Galactoside-binding protein (GALBP), IgE-binding protein, Laminin-binding protein, MAC2, L-29 CPB-35

Background: Galectin-3 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-3 is expressed by a wide range of cell types including activated T cells, tumor cells, macrophages, osteoclasts, fibroblasts and epithelial cells and interacts with β-galactoside sugar moieties. Galectin-3 is associated with cancer, heart failure, stroke and inflammation. Human and mouse Galectin-3 share an 80 % homology by amino acid sequence. Recombinant human Galectin-3 is a non-glycosylated protein, containing 250 amino acids, with a molecular weight of 26 kDa.

UniProt: P17931

Pathways: RTK Signaling