KIT Ligand Protein (KITLG)

Catalog No. ABIN6700200

Supplier Product No.: 012-001-w20-0010

Product Details

Target: KIT Ligand (KITLG)

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 012-001-w20-0010

Supplier: Rockland

Purpose: Rat Stem Cell Factor Recombinant Protein

Purification: Stem Cell Factor purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.

Purity: 98,00%

Endotoxin Level: Measured by LAL is typically ≤ 1 EU/μg protein.

Biological Activity Comment: The activity is determined by its ability to induce proliferation of TF-1 cells and is typically less than 10 ng/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: Stem Cell Factor Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Stem Cell Factor in immunological assays.

Comment:

Suggested_Applications: Cellular Assay

Other_Performance_Data:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: 0.01M acetic acid

Reconstitution_Volume: 10 μL (10-100 μL)

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: KIT Ligand (KITLG)

Alternative Name: Kitlg (KITLG Products)

Background:

Synonyms: c-Kit Ligand, Hematopoietic growth factor KL, KL, Steel Factor, Stem cell factor (SCF), Mast cell growth factor(MGF)

Background: Stem Cell Factor (SCF) is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor known as c-Kit (CD117) and is thought to play a critical role in the maintenance or survival of hematopoietic stem cells. Human SCF shows no activity on murine cells, but murine and rat SCF are active on human cells. Recombinant rat SCF is a non-glycosylated protein, containing 165 amino acids, with a molecular weight of 18.4 kDa.

UniProt: P21581

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway