IL32 Protein
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- Target See all IL32 Proteins
- IL32 (Interleukin 32 (IL32))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-b49
- Supplier
- Rockland
- Purpose
- IL-32A Human Recombinant Protein
- Purification
- Purity was determined to be greater than 97% as determined by reducing and non-reducing SDS- PAGE.
- Purity
- 97,00%
- Endotoxin Level
- Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.
- Biological Activity Comment
- Human IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha production from human PBMCs. The ED50 for this assay is in the range of 0.125- 1.0ug/ml.
- Top Product
- Discover our top product IL32 Protein
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Interleukin 32 (IL32) (AA 1-131) protein (His tag)
IL32 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 32 (IL32) (AA 1-188) protein (GST tag)IL32 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 32 (IL32) (Transcript Variant 8) protein (Myc-DYKDDDDK Tag)IL32 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Interleukin 32 (IL32) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)IL32 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
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Other: User Optimized
Application_Note: IL-32A protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-32A in immunological assays.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- Concentration
- 0.1 mg/mL
- Buffer
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Buffer: 0.05 M Potassium Phosphate, pH 7.8
Stabilizer: None
- Preservative
- Without preservative
- Storage
- -20 °C
- Storage Comment
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
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- Target
- IL32 (Interleukin 32 (IL32))
- Alternative Name
- IL32 (IL32 Products)
- Background
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Synonyms: Interleukin-32 alpha, IL-32a cytokine, Natural killer cells protein 4 alpha, Tumor necrosis factor alpha-inducing factor
Background: Human IL-32 alpha is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-Rbeta. IL-32 alpha has been shown to induce IL-8, TNF-a, and MIP-2 production from human & mouse macrophage cell lines. It is upregulated in activated T- & NK-cells, and IFN-g-treated epithelial cells. Human recombinant IL-32 alpha produced in E. coli is a 131 amino acid non -glycosylated polypeptide with a molecular mass of 14.9 kDa.
- UniProt
- P24001-4
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