IL-27 Protein
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- Target See all IL-27 (IL27) Proteins
- IL-27 (IL27) (Interleukin 27 (IL27))
- Protein Type
- Recombinant
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 010-001-b54
- Supplier
- Rockland
- Purpose
- IL-27/p28 Mouse Recombinant Protein
- Purification
- Purity was determined to be approximately 97% as determined by reducing and non-reducing SDS-PAGE.
- Purity
- 97,00%
- Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein (with a 50% confidence range).
- Biological Activity Comment
- Mouse p28 biological activity was measured via dose-dependent inhibition of TGFß and IL-6-induced IL-17A expression in mouse CD4 splenocytes. 50ng/ml of Mouse p28 is capable of inhibiting >25% of IL-17A expression in this assay. no acceptance criteria limits established.
- Top Product
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Interleukin 27 (IL27) (AA 1-243) protein (GST tag)
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Interleukin 27 (IL27) (AA 177-243) protein (GST tag)IL27 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Interleukin 27 (IL27) protein (His tag)IL27 Origin: Mouse Host: Human Cells Recombinant > 90 % as determined by SDS-PAGE
Interleukin 27 (IL27) proteinIL27 Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, ELISA, IP
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- Application Notes
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Other: User Optimized
Application_Note: IL-27/p28 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-27/p28 in immunological assays.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- Concentration
- 0.1 mg/mL
- Buffer
- Buffer Formulation: 10 mM sodium bicarbonate, pH 8.5.
- Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
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- Target
- IL-27 (IL27) (Interleukin 27 (IL27))
- Alternative Name
- Il27 (IL27 Products)
- Background
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Synonyms: Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A cytokine, IL27-A, p28, IL-30
Background: Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions. Recombinant mouse p28 is a single-chain 207 amino acid peptide with a mass of approximately 23,697 Daltons.
- UniProt
- Q8K3I6
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