IL-25 Protein
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- Target See all IL-25 (IL25) Proteins
- IL-25 (IL25) (Interleukin 25 (IL25))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-v57-0005
- Supplier
- Rockland
- Purpose
- Human Interleukin-17E (IL-25) Recombinant Protein
- Purification
- Interleukin-17E (IL-25) purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
- Purity
- 95,00%
- Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the dose-dependant production of IL-8 from cultured human PBMCs and is typically 10-100 ng/mL.
- Top Product
- Discover our top product IL25 Protein
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Interleukin 25 (IL25) (AA 33-177) protein (His tag)
IL25 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 25 (IL25) (AA 33-177) protein (His-Avi Tag,Biotin)IL25 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 25 (IL25) (Active) proteinIL25 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 25 (IL25) (AA 33-177) protein (Fc Tag)IL25 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 25 (IL25) (AA 17-169) protein (His tag)IL25 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 25 (IL25) (AA 33-177) protein (His tag)IL25 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 25 (IL25) (AA 1-177) protein (GST tag)IL25 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Interleukin 25 (IL25) (Active) protein (Fc Tag)IL25 Origin: Rat Host: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
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- Application Notes
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Other: User Optimized
Application_Note: Interleukin-17E Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-17E in immunological assays.
- Comment
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution_Buffer: 0.01M HCl
Reconstitution_Volume: 5 μL (5-50 μL)
- Buffer
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
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- Target
- IL-25 (IL25) (Interleukin 25 (IL25))
- Alternative Name
- IL25 (IL25 Products)
- Background
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Synonyms: IL17E
Background: Interleukin-17E (IL-17E), also commonly called IL-25, is a pro-inflammatory cytokine member of a six-species family of proteins (IL-17A-17F). IL-17F stimulates secretion of IL-8 and induces activation of NF-kB by binding to the receptor IL-17RB. Recombinant human IL-17E is a non-glycosylated, disulfide-linked, homodimer, containing two 145 amino acid chains, with a total molecular weight of 35.5 kDa.
- UniProt
- Q9H293
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