Interleukin 17a Protein

Catalog No. ABIN6700051

Supplier Product No.: 009-001-b32

Product Details

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Supplier Product No.: 009-001-b32

Supplier: Rockland

Purpose: IL-17A Human Recombinant Protein

Purification: Recombinant protein corresponds to amino acids 19 - 155 of mature human IL-17A. Its molecular weight is 15,667 Daltons. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.

Purity: 90,00%

Endotoxin Level: Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.

Biological Activity Comment: Human IL-17A is fully biologically active when compared to standards via dose-dependent induction of IL-6 production in cultured mouse NIH 3T3 fibroblasts. The ED50 for recombinant human IL-17A in this assay was 1.4-2.1 ng/mL.

Application Details

Application Notes:

Other: User Optimized

Application_Note: IL-17A protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-17A in immunological assays.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 250 μL

Concentration: 0.1 mg/mL

Buffer:

Buffer: 0.1 % Trifluoroacetic acid

Stabilizer: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.

Expiry Date: 6 months

Target Details

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: IL17A (IL17A Products)

Background:

Synonyms: Interleukin-17 cytokine, IL-17, IL-17A, Interleukin-17A, Cytotoxic T-Lymphocyte-associated Antigen 8, CTLA8.

Background: Interleukin17-A is a proinflammatory cytokine produced by activated T cells and exists as a homodimer. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. IL-17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. IL17-A is the founding member of a group of cytokines called the IL-17 family. IL17-A was originally identified as a transcript from a rodent T-cell hybridoma. To elicit its functions, IL17 binds to a type I cell surface receptor called IL17R of which there are at least three variants: IL17RA, IL17RB, and IL17RC.

UniProt: Q16552