IL12 Protein
-
- Target See all IL12 Proteins
- IL12 (Interleukin 12 (IL12))
- Protein Type
- Recombinant
-
Origin
- Mouse
-
Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 010-001-v51-0010
- Supplier
- Rockland
- Purpose
- Mouse Interleukin-12 Recombinant Protein
- Purification
- Interleukin-12 purity was determined to be greater than 95% as determined by reducing and non-reducing SDS-pAGE.
- Purity
- 95,00%
- Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the induced expression of CD25 on CD8+ mouse T cells and is typically detectable starting at 0.1 ng/mL.
- Top Product
- Discover our top product IL12 Protein
-
-
- Application Notes
-
Other: User Optimized
Application_Note: Interleukin-12 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-12 in immunological assays.
- Comment
-
Suggested_Applications: Cellular Assay
Other_Performance_Data:
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- Buffer
- Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.
- Preservative
- Without preservative
- Storage
- -20 °C
- Storage Comment
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
-
- Target
- IL12 (Interleukin 12 (IL12))
- Alternative Name
- Il12a/Il12b (IL12 Products)
- Background
-
Synonyms: NKSF, CLMF
Background: Interleukin-12 (IL-12) is an important mediator of cellular-immunity produced by dendritic cells, macrophages and B cells when stimulated with antigen. IL-12 is involved in differentiating precursor helper T cells to Th1 and/or Th2 subtypes and promotes the production of IFN gamma and TNF alpha. Mouse IL-12 is active on human and mouse cells. Recombinant mouse IL-12 is a glycosylated, disulfide-linked heterodimer. It is containing one p35 su
- UniProt
- P43432
- Pathways
- JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
-