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IL12 Protein

Rockland 010-001-v51-0010 IL12 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 95,00% SDS
Rockland
Catalog No. ABIN6700020
Supplier Product No.: 010-001-v51-0010
  • Target See all IL12 Proteins
    IL12 (Interleukin 12 (IL12))
    Protein Type
    Recombinant
    Origin
    • 20
    • 9
    • 4
    • 1
    Mouse
    Source
    • 14
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Supplier Product No.
    010-001-v51-0010
    Supplier
    Rockland
    Purpose
    Mouse Interleukin-12 Recombinant Protein
    Purification
    Interleukin-12 purity was determined to be greater than 95% as determined by reducing and non-reducing SDS-pAGE.
    Purity
    95,00%
    Endotoxin Level
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is determined by the induced expression of CD25 on CD8+ mouse T cells and is typically detectable starting at 0.1 ng/mL.
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    Discover our top product IL12 Protein
  • Application Notes

    Other: User Optimized

    Application_Note: Interleukin-12 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-12 in immunological assays.

    Comment

    Suggested_Applications: Cellular Assay

    Other_Performance_Data:

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 10 μL (10-100 μL)

    Buffer
    Lyophilized in 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.
    Preservative
    Without preservative
    Storage
    -20 °C
    Storage Comment
    Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Expiry Date
    6 months
  • Target
    IL12 (Interleukin 12 (IL12))
    Alternative Name
    Il12a/Il12b (IL12 Products)
    Background

    Synonyms: NKSF, CLMF

    Background: Interleukin-12 (IL-12) is an important mediator of cellular-immunity produced by dendritic cells, macrophages and B cells when stimulated with antigen. IL-12 is involved in differentiating precursor helper T cells to Th1 and/or Th2 subtypes and promotes the production of IFN gamma and TNF alpha. Mouse IL-12 is active on human and mouse cells. Recombinant mouse IL-12 is a glycosylated, disulfide-linked heterodimer. It is containing one p35 su

    UniProt
    P43432
    Pathways
    JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
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