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IL1A Protein

Rockland 009-001-v24-0010 IL1A Origin: Human Host: Escherichia coli (E. coli) Recombinant 97,00% SDS
Rockland
Catalog No. ABIN6699985
Supplier Product No.: 009-001-v24-0010
  • Target See all IL1A Proteins
    IL1A (Interleukin 1 alpha (IL1A))
    Protein Type
    Recombinant
    Origin
    • 16
    • 10
    • 7
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Source
    • 37
    • 13
    • 11
    • 2
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Supplier Product No.
    009-001-v24-0010
    Supplier
    Rockland
    Purpose
    Human Interleukin-1-alpha Recombinant Protein
    Purification
    Interleukin-1-alpha purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
    Purity
    97,00%
    Endotoxin Level
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is determined by the stimulation of D10S.G4.1 cells and is typically 1-6 pg/mL.
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    Discover our top product IL1A Protein
  • Application Notes

    Other: User Optimized

    Application_Note: Interleukin-1-alpha Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interleukin-1-alpha in immunological assays.

    Comment

    Suggested_Applications: Cellular Assay

    Other_Performance_Data:

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 10 μL (10-100 μL)

    Buffer

    Buffer: 0.01 M Sodium Phosphate, pH 7.5

    Stabilizer: None

    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Expiry Date
    6 months
  • Target
    IL1A (Interleukin 1 alpha (IL1A))
    Alternative Name
    IL1A (IL1A Products)
    Background

    Synonyms: IL-1F1, FAF, BAF, LEM, LAF, Hematopoietin-1

    Background: Interleukin-1 alpha (IL-1α) is constitutively expressed by epithelial cells, but can also be produced by most other cells upon stimulation. IL-1β and IL-1α are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1α is cell associated and IL-1β is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. Recombinant human IL-1α is a non-glycosylated single chain protein, containing 159 amino acids, with a molecular weight of 18 kDa.

    UniProt
    P01583
    Pathways
    NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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