IFNG1-2 Protein
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- Target See all IFNG1-2 products
- IFNG1-2 (Interferon, gamma 1-2 (IFNG1-2))
- Protein Type
- Recombinant
- Origin
- Rat
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Supplier Product No.
- 012-001-v20-0020
- Supplier
- Rockland
- Purpose
- Rat Interferon gamma Recombinant Protein
- Purification
- Interferon gamma purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
- Purity
- 95,00%
- Endotoxin Level
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined in a viral challenge assay using EMC virus on L929 and is typically less than 0.1 ng/mL.
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Interferon, gamma 1-2 (IFNG1-2) protein
IFNG1-2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 95,00%
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- Application Notes
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Other: User Optimized
Application_Note: Interferon-gamma Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interferon-gamma in immunological assays.
- Comment
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 20 μL (20-200 μL)
- Concentration
- 0.1 mg/mL
- Buffer
- Lyophilized in 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.
- Preservative
- Without preservative
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- Expiry Date
- 6 months
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- Target
- IFNG1-2 (Interferon, gamma 1-2 (IFNG1-2))
- Alternative Name
- INF gamma (IFNG1-2 Products)
- Background
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Synonyms: Type II interferon, T cell interferon, Immune Interferon, MAF
Background: Interferon gamma, IFNγ, is a major immune-modulating molecule produced mainly by T cells and natural killer cells activated by antigens, mitogens or alloantigens. Most immune cells express IFNγ receptors and respond to IFNγ-induced signaling by up-regulating MHC class I expression. Recombinant rat IFNγ is a non-glycosylated protein, containing 135 amino acids, with a molecular weight of 15.6 kDa.
- UniProt
- P01581
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