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IFNG1-2 Protein

Rockland 010-001-v20-0020 IFNG1-2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 95,00%
Rockland
Catalog No. ABIN6699978
Supplier Product No.: 010-001-v20-0020
  • Target See all IFNG1-2 products
    IFNG1-2 (Interferon, gamma 1-2 (IFNG1-2))
    Protein Type
    Recombinant
    Origin
    Mouse
    Source
    • 1
    Escherichia coli (E. coli)
    Supplier Product No.
    010-001-v20-0020
    Supplier
    Rockland
    Purpose
    Mouse Interferon gamma Recombinant Protein
    Purification
    Interferon gamma purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
    Purity
    95,00%
    Endotoxin Level
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is determined by Viral CPE assay using EMC virus on L929 cells and is typically greater than 1.0 x 10^7units/mg.
  • Application Notes

    Other: User Optimized

    Application_Note: Interferon-gamma Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Interferon-gamma in immunological assays.

    Comment

    Suggested_Applications: Cellular Assay

    Other_Performance_Data:

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 20 μL (20-200 μL)

    Buffer
    Product is provided as a dried powder, lyophilized from 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.
    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Expiry Date
    6 months
  • Target
    IFNG1-2 (Interferon, gamma 1-2 (IFNG1-2))
    Alternative Name
    INF gamma (IFNG1-2 Products)
    Background

    Synonyms: Type II interferon, T cell interferon, Immune Interferon, MAF

    Background: Interferon gamma, IFNγ, is a major immune-modulating molecule produced mainly by T cells and natural killer cells activated by antigens, mitogens or alloantigens. Most immune cells express IFNγ receptors and respond to IFNγ-induced signaling by up-regulating MHC class I expression. Human IFNγ does not show cross-reactivity with mouse. Recombinant mouse IFNγ is a non-glycosylated protein, containing 134 amino acids, with a molecular weight of 15.6 kDa.

    UniProt
    P01580
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