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GDF5 Protein

Rockland 009-001-u94-0010 GDF5 Origin: Human Host: Escherichia coli (E. coli) Recombinant 98,00% SDS
Rockland
Catalog No. ABIN6699912
Supplier Product No.: 009-001-u94-0010
  • Target See all GDF5 Proteins
    GDF5 (Growth Differentiation Factor 5 (GDF5))
    Protein Type
    Recombinant
    Origin
    • 9
    • 4
    • 2
    Human
    Source
    • 14
    • 1
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Supplier Product No.
    009-001-u94-0010
    Supplier
    Rockland
    Purpose
    Human Growth and Differentiation Factor-5 Recombinant Protein
    Purification
    Growth and Differentiation Factor-5 purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
    Purity
    98,00%
    Endotoxin Level
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is determined by the ability to induce alkaline phosphatase activity in ATDC5 cells is typically 0.1-1 μg/mL.
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  • Application Notes

    Other: User Optimized

    Application_Note: Growth and Differentiation Factor-5 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Growth and Differentiation Factor-5 in immunological assays.

    Comment

    Suggested_Applications: Cellular Assay

    Other_Performance_Data:

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 10 μL (10-100 μL)

    Buffer

    Buffer: 0.1 % Trifluoroacetic acid

    Stabilizer: None

    Preservative
    Without preservative
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    Expiry Date
    6 months
  • Target
    GDF5 (Growth Differentiation Factor 5 (GDF5))
    Alternative Name
    GDF5 (GDF5 Products)
    Background

    Synonyms: Cartilage-derived morphogenetic protein 1

    Background: Growth/Differentiation Factor 5 (GDF-5) is a growth factor that regulates cell proliferation and differentiation in embryonic and adult tissues. GDF-5 is part of the TGF family of proteins and is closely related to the BMP family of proteins. Recombinant human GDF-5 is a non-glycosylated homodimer, containing two 120 amino acids chains, with a total molecular weight of 27.4 kDa. To enable bacterial expression the N-terminal sequence of Ala-Pro-Leu-Thr was replaced with a Lys.

    UniProt
    P43026
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